Immunochemical Analysis of Triton X-100-Insoluble Residues from Micrococcus lysodeikticus Membranes
نویسندگان
چکیده
منابع مشابه
Immunochemical analysis of respiratory-chain components of micrococcus luteus (lysodeikticus).
Membrane-bound antigens of the respiratory chain of Micrococcus luteus were analyzed by crossed immunoelectrophoresis after growth of the organism in the presence of 59Fe, the flavin adenine dinucleotide-flavin mononucleotide precursor D-[2-14C]riboflavin, or the heme precursor 5-amino-[4-(14)C]levulinic acid. Using zymograms and procedures of selective extraction in conjunction with autoradiog...
متن کاملImmunological properties of Micrococcus lysodeikticus membranes.
Membranes of Micrococcus lysodeikticus possess antigens which are distinct from other cellular components such as cytoplasm, ribosomes, and cell walls. Only a few (two to three) components are found when dissociated membranes are examined by immunodiffusion and immunoelectrophoresis techniques. Membranes treated with 0.3% sodium dodecyl sulfate, 0.3% Triton X-100, trypsin, phospholipase A or C,...
متن کاملMembrane Fractions from Micrococcus lysodeikticus
1. A method is described for the isolation of pure mesosomal membrane fractions from Micrococcus lysodeikticus. 2. Plasmolysis of cells, before wall digestion, was necessary for effective mesosome release. 3. The effect of mild shearing forces, temperature and time upon the release of mesosomal membrane from protoplasts was investigated. 4. The optimum yield of mesosomal membranes from stable p...
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Addition of Triton X-100 to chloroplast suspensions to a final concentration of 100-200 microM causes an approximate tripling of chloroplast volume and complete inhibition of light-induced conformational changes, light-dependent hydrogen ion transport, and photophosphorylation. Electron microscopic studies show that chloroplasts treated in this manner manifest extensive swelling in the form of ...
متن کاملPhotocatalytic Degradation of Triton X-100 by Zinc oxide Nanoparticles
In this study the application of ZnO nanoparticles to UV photocatalytic degradation of nonionic surfactantTriton X-100 in aqueous media was investigated. The affecting factors on the photodegradation such as TritonX-100 initial concentration, nanocatalyst weight, pH, temperature and other parameters were studied anddescribed in details. The degradation rate was found to be strongly influenced b...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1979
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.140.3.881-887.1979